SPC25 antibody (AA 121-224)

Catalog No. ABIN872594

The Rabbit Polyclonal anti-SPC25 antibody is suitable to detect SPC25 in samples from Mouse. It has been validated for WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p).

Quick Overview for SPC25 antibody (AA 121-224) (ABIN872594)

Target: SPC25 (SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPC25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SPC25 Antibody

Binding Specificity: AA 121-224

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SPC25

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SPC25

Target: SPC25 (SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25))

Alternative Name: SPC25

Background:

Synonyms: AD024, hSpc25, Kinetochore protein Spc25, SPBC25, SPC25, SPC25_HUMAN, spindle pole body component 25 homolog.

Background: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Gene ID: 57405