CKM antibody (AA 221-330)

Catalog No. ABIN872835

This anti-CKM antibody is a Rabbit Polyclonal antibody detecting CKM in WB, ELISA, ICC, IHC (p), IF (p), IF (cc) and IHC (fro). Suitable for Mouse, Rat and Zebrafish (Danio rerio). This Primary Antibody has been cited in 1 publication.

Quick Overview for CKM antibody (AA 221-330) (ABIN872835)

Target: CKM (Creatine Kinase, Muscle (CKM))

Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CKM Antibody

Binding Specificity: AA 221-330

Cross-Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Creatine Kinase MM

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CKM antibody (ABIN872835)

Rueda, Johnson, Giddabasappa, Swaroop, Brooks, Sigel, Chaney, Fox: "The cellular and compartmental profile of mouse retinal glycolysis, tricarboxylic acid cycle, oxidative phosphorylation, and ~P transferring kinases." in: Molecular vision, Vol. 22, pp. 847-85, (2016) (PubMed).

Target Details for CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Alternative Name: Creatine Kinase MM

Background:

Synonyms: CKMM, M-CK, Creatine kinase M-type, Creatine kinase M chain, CKM

Background: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Gene ID: 1158

UniProt: P06732