SKA3 antibody (AA 201-300)

Catalog No. ABIN872869

This Rabbit Polyclonal antibody specifically detects SKA3 in ELISA, WB, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human and Ratand has been mentioned in 1 publication.

Quick Overview for SKA3 antibody (AA 201-300) (ABIN872869)

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SKA3 Antibody

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SKA3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SKA3 antibody (ABIN872869)

Hou, Wang, Huang, Sun, Zhao, Shi, Li, Wang, He, Tam, Wu: "SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma." in: Cell death & disease, Vol. 10, Issue 12, pp. 929, (2020) (PubMed).

Target Details for SKA3

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Alternative Name: SKA3

Background:

Synonyms: C13orf3, RAMA1, SKA3, SKA3_HUMAN, Spindle and kinetochore associated complex subunit 3, Spindle and kinetochore-associated protein 3.

Background: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.