Crossover junction endonuclease EME1 (EME1) (AA 412-500) antibody

Catalog No. ABIN873042

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, ELISA, IHC (fro), IHC (p), IF (cc) and IF (p). Suitable for Human.

Quick Overview for Crossover junction endonuclease EME1 (EME1) (AA 412-500) antibody (ABIN873042)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: AA 412-500

Predicted Reactivity: Human,Mouse,Rat,Dog,Sheep,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EME1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1

Background:

Synonyms: Crossover junction endonuclease EME1, Eme1 essential meiotic endonuclease 1 homolog 1 S. pombe, EME1, S. pombe, homolog of, 1, essential meiotic endonuclease 1 homolog 1 S pombe, Essential Meiotic Endonuclease 1 Homolog 1, essential meiotic endonuclease 1 homolog 2, Essential meiotic endonuclease 1, S. pombe, homolog of, 1, hMMS4, homolog of yeast EME1 endonuclease, MMS4, MMS4 homolog, MMS4L, EME1_HUMAN.

Background: EME1 complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein (MUS81) to form an endonuclease complex which cleaves branched DNA structures, especially those arising during stalled DNA replication. The protein may be involved in repairing DNA damage and in maintaining genomic stability. It interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. Alternative splicing results in multiple transcript variants.

Gene ID: 146956