Crossover junction endonuclease EME1 (EME1) (N-Term) antibody

Catalog No. ABIN6741209

The Rabbit Polyclonal anti-not_set antibody has been validated for WB. It is suitable to detect not_set in samples from Human, Mouse, Pig, Cow, Horse and Monkey.

Quick Overview for Crossover junction endonuclease EME1 (EME1) (N-Term) antibody (ABIN6741209)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human, Mouse, Pig, Cow, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Specificity: Human Eme1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Pig (100%) Rat, Dog, Guinea pig (92%) Horse (85%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human EME1 (Q96AY2, NP_689676). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Pig (100%), Gorilla, Rat, Panda, Dog, Guinea pig (92%), Rabbit, Horse (85%), Bovine (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1

Background: Name/Gene ID: EME1

Synonyms: EME1, MMS4, MMS4L, MMS4 homolog, SLX2A, HMMS4

Gene ID: 146956

NCBI Accession: NP_689676

UniProt: Q96AY2