SETDB2 antibody (AA 21-120)

Catalog No. ABIN873188

The Rabbit Polyclonal anti-SETDB2 antibody has been validated for WB, ELISA, IF (cc), IF (p), ICC, IHC (fro) and IHC (p). It is suitable to detect SETDB2 in samples from Rat.

Quick Overview for SETDB2 antibody (AA 21-120) (ABIN873188)

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SETDB2 Antibody

Binding Specificity: AA 21-120

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CLLD8/SETDB2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SETDB2

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Alternative Name: CLLD8

Background:

Synonyms: Histone-lysine N-methyltransferase SETDB2, C13orf4, Chromosome 13 open reading frame 4, Chronic lymphocytic leukemia deletion region 8, Chronic lymphocytic leukemia deletion region gene 8, Chronic lymphocytic leukemia deletion region gene 8 protein, Clld8, CLLL8, Gm293, H3-K9-HMTase, Histone H3-K9 methyltransferase, KMT1F, Lysine N-methyltransferase 1F, Probable histone-lysine N-methyltransferase H3 lysine-9 specic, SEB2, SET domain burcated 2, SET domain protein, burcated, 2, SETB2_HUMAN, Setdb2.

Background: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

Gene ID: 83852