DBF4 antibody (AA 21-110)

Catalog No. ABIN873251

The Rabbit Polyclonal anti-DBF4 antibody is suitable to detect DBF4 in samples from Human. It has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro).

Quick Overview for DBF4 antibody (AA 21-110) (ABIN873251)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DBF4 Antibody

Binding Specificity: AA 21-110

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DBF4A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: DBF4A

Background:

Synonyms: Activator of S phase kinase, ASK, Chfon homolog A, DBF4, DBF4 type zinc finger containing protein 1, DBF4-type zinc finger-containing protein 1, DBF4A, DBF4A_HUMAN, Protein DBF4 homolog A, ZDBF1.

Background: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.Tissue specificity: Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

Gene ID: 10926

Pathways: Mitotic G1-G1/S Phases, DNA Replication