ACLY antibody (pSer455) (AbBy Fluor® 647)

Catalog No. ABIN876240

The AbBy Fluor® 647-conjugated Rabbit Polyclonal anti-ACLY antibody (ABIN876240) specifically detects ACLY in WB, IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for ACLY antibody (pSer455) (AbBy Fluor® 647) (ABIN876240)

Target: ACLY (ATP Citrate Lyase (ACLY))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACLY antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ACLY Antibody (AbBy Fluor® 647)

Binding Specificity: pSer455

Purpose: phospho-ACLY (Ser455) Polyclonal Antibody, AbBy Fluor-647 Conjugated

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ATP citrate lyase around the phosphorylation site of Ser455

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ATP citrate lyase

Background:

Synonyms: ATP citrate lyase phospho S455, ACL, ATP citrate pro-S lyase, ATP citrate synthase, Citrate cleavage enzyme, EC 2.3.3.8, A730098H14RIK, ACLY, ATP CITRATE LYASE, ATPCL, AW538652, Cce, CITRATE LYASE, CLATP, MGC124629.

Background: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Gene ID: 47

Pathways: Warburg Effect