Ephrin B1 antibody (pTyr324, pTyr329) (AbBy Fluor® 555)

Catalog No. ABIN879516

This anti-Ephrin B1 antibody is a Rabbit Polyclonal antibody detecting Ephrin B1 in WB, IF (p) and IF (cc). Suitable for Human.

Quick Overview for Ephrin B1 antibody (pTyr324, pTyr329) (AbBy Fluor® 555) (ABIN879516)

Target: Ephrin B1 (EFNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Ephrin B1 Antibody (AbBy Fluor® 555)

Binding Specificity: pTyr324, pTyr329

Specificity: These phosphorylation sites are homologous to Tyr323 + Tyr328 in Mouse.

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr324/329

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: EFNB1

Background:

Synonyms: CFND, CFNS, EFB1, EFL3, EPLG2, Elk-L, LERK2, Ephrin-B1, EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, LERK-2, EFNB1

Background: Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity). Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

Gene ID: 1947

UniProt: P98172

Pathways: RTK Signaling