ATG12 antibody (AA 101-187) (AbBy Fluor® 488)

Catalog No. ABIN882997

This Rabbit Polyclonal antibody specifically detects ATG12 in WB, IF (cc) and IF (p). It exhibits reactivity toward Mouse and Rat.

Quick Overview for ATG12 antibody (AA 101-187) (AbBy Fluor® 488) (ABIN882997)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ATG12 Antibody (AbBy Fluor® 488)

Binding Specificity: AA 101-187

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATG12

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: Apg12

Background:

Synonyms: APG12, FBR93, APG12L, HAPG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12

Background: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG1 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.

Gene ID: 9140

UniProt: O94817

Pathways: Autophagy