BUB3 antibody (AA 201-300) (FITC)

Catalog No. ABIN885811

This Rabbit Polyclonal antibody specifically detects BUB3 in WB, IF (cc) and IF (p). It exhibits reactivity toward Rat.

Quick Overview for BUB3 antibody (AA 201-300) (FITC) (ABIN885811)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-BUB3 Antibody (FITC)

Binding Specificity: AA 201-300

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Bub3

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: Bub3

Background:

Synonyms: BUB3L, hBUB3, Mitotic checkpoint protein BUB3, BUB3

Background: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Gene ID: 9184

UniProt: O43684

Pathways: Maintenance of Protein Location