Cyclin B1 antibody (AA 271-433) (AbBy Fluor® 488)

Catalog No. ABIN892886

The Rabbit Polyclonal anti-Cyclin B1 antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect Cyclin B1 in samples from Human, Mouse, Rat, Cow and Chicken.

Quick Overview for Cyclin B1 antibody (AA 271-433) (AbBy Fluor® 488) (ABIN892886)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cyclin B1 Antibody (AbBy Fluor® 488)

Binding Specificity: AA 271-433

Cross-Reactivity: Chicken, Cow, Human, Mouse, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CCNB1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background:

Synonyms: CycB1, Cycb-4, Cycb-5, Ccnb1-rs1, Cycb1-rs1, Ccnb1-rs13, G2/mitotic-specific cyclin-B1, Ccnb1, Ccn-2, Cycb

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Gene ID: 268697

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase