DBF4 antibody (AA 21-110) (HRP)

Catalog No. ABIN893539

This Rabbit Polyclonal antibody specifically detects DBF4 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for DBF4 antibody (AA 21-110) (HRP) (ABIN893539)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DBF4 Antibody (HRP)

Binding Specificity: AA 21-110

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DBF4A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: DBF4A

Background:

Synonyms: Activator of S phase kinase, ASK, Chfon homolog A, DBF4, DBF4 type zinc finger containing protein 1, DBF4-type zinc finger-containing protein 1, DBF4A, DBF4A_HUMAN, Protein DBF4 homolog A, ZDBF1.

Background: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.Tissue specificity: Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

Gene ID: 10926

Pathways: Mitotic G1-G1/S Phases, DNA Replication