EPH Receptor A4 antibody (AA 531-630) (AbBy Fluor® 555)

Catalog No. ABIN895465

This Rabbit Polyclonal antibody specifically detects EPH Receptor A4 in IF (cc) and IF (p). It exhibits reactivity toward Rat.

Quick Overview for EPH Receptor A4 antibody (AA 531-630) (AbBy Fluor® 555) (ABIN895465)

Target: EPH Receptor A4 (EPHA4)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A4 antibody is conjugated to AbBy Fluor® 555

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-EPH Receptor A4 Antibody (AbBy Fluor® 555)

Binding Specificity: AA 531-630

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EphA4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EPH Receptor A4

Target: EPH Receptor A4 (EPHA4)

Alternative Name: Eph Receptor A4

Background:

Synonyms: SEK, HEK8, TYRO1, Ephrin type-A receptor 4, EPH-like kinase 8, EK8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EPHA4

Background: Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

Gene ID: 2043

UniProt: P54764

Pathways: RTK Signaling