Nucleostemin antibody (AA 101-160) (HRP)

Catalog No. ABIN909189

The HRP-conjugated Rabbit Polyclonal anti-Nucleostemin antibody (ABIN909189) specifically detects Nucleostemin in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for Nucleostemin antibody (AA 101-160) (HRP) (ABIN909189)

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleostemin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Nucleostemin Antibody (HRP)

Binding Specificity: AA 101-160

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nucleostemin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nucleostemin

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Alternative Name: Nucleostemin

Background:

Synonyms: E2 induced gene 3 protein, E2IG3, Estradiol induced nucleotide binding protein, GNL3, Guanine nucleotide binding protein like 3 nucleolar, Guanine nucleotide binding protein like 3, MGC800, NNP47, Novel nucleolar protein 47, NS, Nucleolar GTP binding protein 3, Nucleostemin, Putative nucleotide binding protein estradiol induced, C77032, GNL3_HUMAN.

Background: Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell cycle progression in stem cells and cancer cells.

Gene ID: 26354