PHGDH antibody (AA 101-200) (AbBy Fluor® 350)

Catalog No. ABIN910982

Product Details anti-PHGDH Antibody (AbBy Fluor® 350)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Binding Specificity: AA 101-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHGDH

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH (PHGDH Products)

Background:

Synonyms: NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113, D-3-phosphoglycerate dehydrogenase, PHGDH, PGDH3

Background: The survival and development of central neurons require the supply of trophic factors by glial cells. The trophic actions of glial cells on Purkinje neurons are mediated by L-serine and glycine, which are glia-derived trophic factors synthesized by 3PGDH (1). 3PGDH protein is 544 amino acids in length. Two distinct mRNA transcripts that encode for 3PGDH protein in normal human tissues are dominant 2.1 kb mRNA, which is highly expressed in prostate, testis, ovary, brain, liver, kidney, and pancreas, and weakly expressed in thymus, colon, and heart, and 710 bp mRNA, which is highly expressed in heart and skeletal muscle (2). 3PGDH is regulated at the transcriptional level depending on tissue specificity and cellular proliferative status (2). 3PGDH protein is also highly expressed in adult and fetal brain tissues (3). 3PGDH protein plays an important role in the metabolism, development, and function of the central nervous system (3) and its deficiency is a treatable congential error (4-5) that impairs L-serine biosynthesis which is characterized by congenital microcephaly, psychomotor retardation, and seizures (3).

Gene ID: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect