RFFL antibody (AA 251-363) (Cy3)

Catalog No. ABIN913499

The Rabbit Polyclonal anti-RFFL antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect RFFL in samples from Human and Mouse.

Quick Overview for RFFL antibody (AA 251-363) (Cy3) (ABIN913499)

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-RFFL Antibody (Cy3)

Binding Specificity: AA 251-363

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RING finger protein 189

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RING finger protein 189

Background:

Synonyms: CARP-2, CARP2, Caspase regulator CARP2, Caspases 8 and 10 associated RING finger protein 2, Caspases-8 and -10-associated RING finger protein 2, E3 ubiquitin protein ligase rylin, E3 ubiquitin-protein ligase rylin, Fring, FYVE RING finger protein Sakura, FYVE-RING finger protein Sakura, Rffl, RFFL_HUMAN, Rylin, RING finger and FYVE like domain containing protein, RING finger and FYVE-like domain-containing protein 1, RING finger protein 189, RING finger protein 34 like, RING finger protein 34-like, RNF189, RNF34L.

Background: Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.

Gene ID: 117584