T-Bet antibody (AbBy Fluor® 488)

Catalog No. ABIN917243

The Rabbit Polyclonal anti-T-Bet antibody has been validated for WB and IF (p). It is suitable to detect T-Bet in samples from Human, Mouse and Rat.

Quick Overview for T-Bet antibody (AbBy Fluor® 488) (ABIN917243)

Target: T-Bet

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-T-Bet Antibody (AbBy Fluor® 488)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Tbx21

Isotype: IgG

Application Details

Application Notes: IF(IHC-P): (1:50-200)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for T-Bet

Target: T-Bet

Alternative Name: Tbx21/T-Bet

Background:

Synonyms: T bet, T box 21, T box expressed in T cells, T box protein 21, T box transcription factor TBX21, T cell specic T box transcription factor, T cell specic T box transcription factor T bet, T PET, TBET, TBLYM, TBX 21, TBX21, TPET, Transcription factor TBLYM, TBX21_HUMAN, T-box transcription factor TBX21, T-box protein 21, T-cell-specic T-box transcription factor T-bet, Transcription factor TBLYM.

Background: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008].

Gene ID: 30009