S100A8 antibody

Catalog No. ABIN920692

Product Details anti-S100A8 Antibody

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: M8I-74

Application Details

Application Notes: Immunohistochemistry: For acetone-fixed frozen sections, use at a 1:20 dilution followed by incubation with rabbit anti-rat Ig and a monoclonal rat APAAP complex. Alternatively, after incubation with M8I-74 at a 1:20 dilution and washing, slides can be incubated with biotinylated rabbit anti-rat Ig and streptavidin conjugated to horseradish peroxidase. Immunocytochemistry: For acetone-fixed cytospin preparations, use at a 1:20-1:50 dilution. Western blot: Use at a 1:20-1:50 dilution followed by anti-rat-HRP. Flow cytometry: Optimal conditions still to be determined. MRP8 expression as determined by anti-MRP8, clone M8I-74 HEK Cells HEK Cells transfected with MRP8

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 mL (>200 tests) monoclonal antibody at 250 µg IgG/mL in serum-free culture supernatant with protein stabilizer and 0.1% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: MRP8 (S100A8 Products)

Background: Synonyms: ABCC11

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins