TLR9 antibody (Biotin)
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- Target See all TLR9 Antibodies
- TLR9 (Toll-Like Receptor 9 (TLR9))
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TLR9 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoassay (IA)
- Specificity
- Reacts with mouse TLR9. Weak cross-reactivity with human TLR9.
- Sterility
- 0.2 μm filtered
- Clone
- 5G5
- Isotype
- IgG2a
- Top Product
- Discover our top product TLR9 Primary Antibody
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- Application Notes
- Flow cytometry: Use at a 1:10 dilution. Immunoassays: (detection antibody) Immunohistochemistry: (frozen sections) stains RAW macrophages and TLR9 transfected HEK293 cells
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.5 mL of 100 µg/mL 0.2 µm filtered monoclonal antibody solution in PBS containing protein stabilizer and 0.02% sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- TLR9 (Toll-Like Receptor 9 (TLR9))
- Alternative Name
- TLR9 (TLR9 Products)
- Synonyms
- CD289 antibody, toll like receptor 9 antibody, toll-like receptor 9 antibody, TLR9 antibody, Tlr9 antibody
- Background
- Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defence of many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLR identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signals via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG- dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9.
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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