His Tag antibody (FITC)

Catalog No. ABIN920803

This Chicken Polyclonal antibody specifically detects His Tag in WB and ELISA. It exhibits reactivity toward .

Quick Overview for His Tag antibody (FITC) (ABIN920803)

Target: His Tag

Reactivity: Please inquire

Host: Chicken

Clonality: Polyclonal

Conjugate: This His Tag antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA

Product Details anti-His Tag Antibody (FITC)

Sequence: HHHHHH

Application Details

Application Notes: Western blot: Use at 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 7.2 with 0.2% BSA and 0.1% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for His Tag

Target: His Tag

Alternative Name: Hexahistidine-Tag

Target Type: Tag

Background: Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support and conjugated with fluorescein isothiocyanate (FITC). Specification: Prior to conjugation, the antibodies were analyzed by ELISA and Western blot analyses. The molar F/P ratio is 3.6.