His Tag antibody (HRP)

Catalog No. ABIN920804

The Chicken Polyclonal anti-His Tag antibody has been validated for WB and ELISA. It is suitable to detect His Tag in samples from .

Quick Overview for His Tag antibody (HRP) (ABIN920804)

Target: His Tag

Reactivity: Please inquire

Host: Chicken

Clonality: Polyclonal

Conjugate: This His Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-His Tag Antibody (HRP)

Sequence: HHHHHH

Application Details

Application Notes: Western blot: Use at 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM HEPES pH 7.0, 0.1M Sodium Chloride with 0.2% BSA and 0.01% thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for His Tag

Target: His Tag

Alternative Name: Hexahistidine-Tag

Target Type: Tag

Background: Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support and the antibodies were conjugated with horseradish peroxidase (HRP). Specification: ELISA: HRP-conjugated chicken anti- hexahistidine diluted 1:10,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.