POLR2B antibody (N-Term)

Catalog No. ABIN925777

This Rabbit Polyclonal antibody specifically detects POLR2B in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Xenopus laevis and Chicken.

Quick Overview for POLR2B antibody (N-Term) (ABIN925777)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Xenopus laevis, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2B Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Frog, Chicken

Immunogen: POLR2 B antibody was raised in rabbit using the N terminal of POLR2 as the immunogen

Application Details

Application Notes: WB: 5 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

POLR2B Blocking Peptide, (ABIN5615464), is also available for use as a blocking control in assays to test for specificity of this POLR2B antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 100 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: POLR2B

Background: POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. Synonyms: Polyclonal POLR2B antibody, Anti-POLR2B antibody, polymerase, RNA II, DNA directed polypeptide B, 140kDa antibody.

Pathways: Regulatory RNA Pathways, DNA Damage Repair