Caspase 3 antibody (N-Term)

Catalog No. ABIN925983

This anti-Caspase 3 antibody is a Rabbit Polyclonal antibody detecting Caspase 3 in WB and IHC. Suitable for Human, Rat, Cow, Dog, Rabbit and Pig.

Quick Overview for Caspase 3 antibody (N-Term) (ABIN925983)

Target: Caspase 3 (CASP3)

Reactivity: Human, Rat, Cow, Dog, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Caspase 3 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Rat (Rattus), Pig (Porcine), Dog (Canine), Rabbit, Cow (Bovine)

Immunogen: CASP3 antibody was raised in rabbit using the N terminal of CASP3 as the immunogen

Application Details

Application Notes: WB: 5 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CASP3 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this CASP3 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 100 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Background: CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. Synonyms: Polyclonal CASP3 antibody, Anti-CASP3 antibody, caspase 3, apoptosis-related cysteine peptidase antibody.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation