Hexokinase 1 antibody (N-Term)

Catalog No. ABIN926654

The Rabbit Polyclonal anti-Hexokinase 1 antibody has been validated for WB. It is suitable to detect Hexokinase 1 in samples from Human.

Quick Overview for Hexokinase 1 antibody (N-Term) (ABIN926654)

Target: Hexokinase 1 (HK1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: N-Term

Purification: Purified

Immunogen: HK1 antibody was raised in rabbit using the N terminal of HK1 as the immunogen

Application Details

Application Notes: Optimal conditions should be determined by the investigator.

Comment:

HK1 Blocking Peptide, (ABIN937173), is also available for use as a blocking control in assays to test for specificity of this HK1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: HK1

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus, the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. Synonyms: Polyclonal HK1 antibody, Anti-HK1 antibody, hexokinase 1 antibody, HK1-ta antibody, HK1-tb antibody, HK1-tc antibody, HKI antibody, HXK1 antibody.

Pathways: Carbohydrate Homeostasis, Warburg Effect