TOP2B antibody (Middle Region)

Catalog No. ABIN927425

The Rabbit Polyclonal anti-TOP2B antibody has been validated for WB. It is suitable to detect TOP2B in samples from Human, Mouse and Rat.

Quick Overview for TOP2B antibody (Middle Region) (ABIN927425)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TOP2B Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified

Immunogen: TOP2 B antibody was raised in rabbit using the middle region of TOP2 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

TOP2B Blocking Peptide, (ABIN938296), is also available for use as a blocking control in assays to test for specificity of this TOP2B antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: TOP2B

Background: TOP2B is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. Synonyms: Polyclonal TOP2B antibody, Anti-TOP2B antibody, topoisomerase, DNA II beta 180kDa antibody, TOPIIB antibody, top2beta antibody.