FTO antibody (N-Term)

Catalog No. ABIN928144

Product Details anti-FTO Antibody

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine)

Purification: Purified

Immunogen: Fto antibody was raised in rabbit using the N terminal of Fto as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Fto Blocking Peptide, catalog no. 33R-6164, is also available for use as a blocking control in assays to test for specificity of this Fto antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: Fto (FTO Products)

Background: Fto is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Fto has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Fto has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Fto has no activity towards 1-methylguanine and no detectable activity towards double-stranded DNA. Fto requires molecular oxygen, alpha-ketoglutarate and iron. Fto contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Synonyms: Polyclonal Fto antibody, Anti-Fto antibody, fat mass and obesity associated antibody, AW743446 antibody, mKIAA1752 antibody.