HELT antibody (Middle Region)

Catalog No. ABIN928349

The Rabbit Polyclonal anti-HELT antibody has been validated for WB. It is suitable to detect HELT in samples from Mouse, Human, Rat, Cow and Zebrafish (Danio rerio).

Quick Overview for HELT antibody (Middle Region) (ABIN928349)

Target: HELT (Helt BHLH Transcription Factor (HELT))

Reactivity: Mouse, Human, Rat, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HELT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HELT Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Zebrafish (Brachydanio rerio), Cow (Bovine)

Purification: Purified

Immunogen: HELT antibody was raised in rabbit using the middle region of HELT as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

HELT Blocking Peptide, (ABIN937981), is also available for use as a blocking control in assays to test for specificity of this HELT antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for HELT

Target: HELT (Helt BHLH Transcription Factor (HELT))

Alternative Name: HELT

Background: HELT belongs to the HEY family. It contains 1 basic helix-loop-helix (bHLH) domain and 1orange domain. HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'. Synonyms: Polyclonal HELT antibody, Anti-HELT antibody, HES/HEY-like transcription factor antibody, HCM1228 antibody, HESL antibody, Mgn antibody.

Pathways: Feeding Behaviour