PROX1 antibody (C-Term)

Catalog No. ABIN929458

This Rabbit Polyclonal antibody specifically detects PROX1 in WB, IF and IHC (p). It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for PROX1 antibody (C-Term) (ABIN929458)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PROX1 Antibody

Binding Specificity: C-Term

Specificity: This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by immunostaining, and human Prox 1 by western blot.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Equal reactivity with Human, Mouse, and Rat

Purification: Protein A affinity chromatography

Immunogen: Prox 1 antibody was raised in rabbit using recombinant human C-terminal part of Prox-1 (homeo domain and prospero domain) as the immunogen

Isotype: IgG

Application Details

Application Notes: WB: 1-5µg/mL. For other applications, optimal conditions should be determined by end-user
Optimal conditions should be determined by the investigator.

Comment:

The lyophilized IgG is stable at 4 °C for at least one month and for more than a year when kept at -20 °C. When reconstituted in sterile water to a concentration of >0.5 mg/mL the antibody is stable for at least six weeks at 2-4 °C in the presence of a preservative.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: IgG fraction supplied lyophilizedfrom PBS, pH 7.4 with no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized antibody at -20 °C. Following reconstitution store at 4 °C in the short-term. For longer term storage aliquot and store at -20 °C or -70 °C.

References for anti-PROX1 antibody (ABIN929458)

Zhang, Brady, Liang, Wu, Henkemeyer, Yan: "EphB4 forward signalling regulates lymphatic valve development." in: Nature communications, Vol. 6, pp. 6625, (2016) (PubMed).

Kulkarni, Herman, Ikegami, Greenberg, Akeson: "Lymphatic ontogeny and effect of hypoplasia in developing lung." in: Mechanisms of development, Vol. 128, Issue 1-2, pp. 29-40, (2011) (PubMed).

Kulkarni, Greenberg, Akeson: "NFATc1 regulates lymphatic endothelial development." in: Mechanisms of development, Vol. 126, Issue 5-6, pp. 350-65, (2009) (PubMed).

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: Prox 1

Background: Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lympathic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. Synonyms: Polyclonal Prox 1 antibody, Anti-Prox 1 antibody, Prox-1 antibody, Prospero related homeobox 1 antibody, Homeobox prospero like protein antibody, Homeobox prospero like protein PROX1 antibody, Prospero homeobox protein 1 antibody, Prospero related homeobox 1 antibody.

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation