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Actin antibody (N-Term)

This anti-Actin antibody is a Mouse Monoclonal antibody detecting Actin in WB, IHC and IF. Suitable for Cow.
Catalog No. ABIN930825

Quick Overview for Actin antibody (N-Term) (ABIN930825)

Target

See all Actin Antibodies
Actin

Reactivity

  • 102
  • 78
  • 74
  • 27
  • 21
  • 20
  • 12
  • 11
  • 5
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cow

Host

  • 88
  • 41
  • 3
  • 1
  • 1
  • 1
Mouse

Clonality

  • 80
  • 55
Monoclonal

Conjugate

  • 78
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This Actin antibody is un-conjugated

Application

  • 107
  • 41
  • 32
  • 29
  • 28
  • 27
  • 25
  • 22
  • 18
  • 16
  • 15
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone

ASM-1
  • Binding Specificity

    • 16
    • 15
    • 14
    • 12
    • 10
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Cross-Reactivity

    Cow (Bovine), Horse (Equine), Rat (Rattus), Mouse (Murine), Chicken

    Purification

    Protein A affinity chromatography

    Immunogen

    Actin antibody was raised in mouse using synthetic N- terminus decapeptide of alpha-smooth-muscle isoform of actin as the immunogen.

    Isotype

    IgG2a
  • Application Notes

    Optimal conditions should be determined byt he investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Supplied in liquid form, contains 0.09 % NaN3 and 0.5 % BSA in PBS pH 7.4

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze/thaw cycles.
    Dilute only prior to immediate use.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at 2-8 °C for short term storage. Aliquot and store at -20 °C for long term storage.
  • Target

    Actin

    Background

    Actin is a globular, roughly 42-kDa moonlighting protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Synonyms: Monoclonal Smooth Muscle Actin antibody, Anti Smooth Muscle Actin antibody.
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