RPF2 antibody

Catalog No. ABIN930978

The Mouse Monoclonal anti-RPF2 antibody (Clone 2099C7a) (ABIN930978) specifically detects RPF2 in WB, DB and ICC. The antibody is reactive with Human samples.

Quick Overview for RPF2 antibody (ABIN930978)

Target: RPF2 (Ribosome Production Factor 2 Homolog (RPF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPF2 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB), Immunocytochemistry (ICC)

Clone: 2099C7a

Product Details anti-RPF2 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: BXDC1 antibody was raised in mouse using recombinant Human Brix Domain Containing 1 (Bxdc1)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: BXDC1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for RPF2

Target: RPF2 (Ribosome Production Factor 2 Homolog (RPF2))

Alternative Name: BXDC1

Background: BXDC1 (Brix domain-containing protein 1) is a 306 amino acid protein encoded by the human gene BXDC1. BXDC1 is a nuclear protein that contains one Brix domain. Brix domain-containing proteins represent a family of proteins involved in the biogenesis of large ribosomal subunits. The Brix domain is a region with homology to the yeast protein Pitx1 (ribosome biogenesis protein BRX1). Pitx1 is part of a complex that includes RPF1, RPF2 and SSF1 or SSF2. This complex is required for the biogenesis of the 60S ribosomal subunit. Synonyms: Monoclonal BXDC1 antibody, Anti-BXDC1 antibody, Ribosome production factor 2 homolog antibody, RPF2 antibody, FLJ21087 antibody, bA397G5.4 antibody.

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly