SAE1 antibody

Catalog No. ABIN931206

The Mouse Monoclonal anti-SAE1 antibody (Clone 2946C4a) (ABIN931206) specifically detects SAE1 in ICC and DB. The antibody is reactive with Human samples.

Quick Overview for SAE1 antibody (ABIN931206)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAE1 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 2946C4a

Product Details anti-SAE1 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: SAE1 antibody was raised in mouse using recombinant Human Sumo1 Activating Enzyme Subunit 1 (Sae1)

Isotype: IgG1

Application Details

Application Notes: ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: SAE1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1

Background: The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. Synonyms: Monoclonal SAE1 antibody, Anti-SAE1 antibody, SUMO-activating enzyme subunit 1 antibody, AOS1 antibody, SUA1 antibody, FLJ3091 antibody, HSPC140 antibody.