HIRA antibody

Catalog No. ABIN932202

The Mouse Monoclonal anti-HIRA antibody (Clone 374C6a) (ABIN932202) specifically detects HIRA in ICC and DB. The antibody is reactive with Human samples.

Quick Overview for HIRA antibody (ABIN932202)

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 374C6a

Product Details anti-HIRA Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: TUPLE1 antibody was raised in mouse using recombinant H.Sapiens Tup1-Like Enhancer Of Split Gene 1 (Tuple1)

Isotype: IgG2a

Application Details

Application Notes: ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: TUPLE1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: TUPLE1

Background: This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. Synonyms: Monoclonal TUPLE1 antibody, Anti-TUPLE1 antibody, TUP1-like enhancer of split protein 1 antibody.

Pathways: Chromatin Binding