PRPF8 antibody

Catalog No. ABIN933118

This Mouse Monoclonal antibody specifically detects PRPF8 in WB, ICC, FACS and DB. It exhibits reactivity toward Human.

Quick Overview for PRPF8 antibody (ABIN933118)

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRPF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Dot Blot (DB)

Clone: 2834C1a

Product Details anti-PRPF8 Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: PRPF8 antibody was raised in mouse using recombinant Human Prp8 Pre- Processing Factor 8 Homolog (Yeast) (Prpf8)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: PRPF8 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for PRPF8

Target: PRPF8 (PRP8 Pre-mRNA Processing Factor 8 (PRPF8))

Alternative Name: PRPF8

Background: Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. Synonyms: Monoclonal PRPF8 antibody, Anti-PRPF8 antibody, PRP8 pre-mRNA processing factor 8 homolog antibody, PRP8 antibody, RP13 antibody, HPRP8 antibody, PRPC8 antibody.