NFS1 antibody

Catalog No. ABIN933137

The Mouse Monoclonal anti-NFS1 antibody is suitable to detect NFS1 in samples from Human. It has been validated for WB and DB.

Quick Overview for NFS1 antibody (ABIN933137)

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFS1 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Clone: 2635E1a

Product Details anti-NFS1 Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: NFS1 antibody was raised in mouse using recombinant Human Nfs1 Nitrogen Fixation 1 Homolog (S. Cerevisiae)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: NFS1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for NFS1

Target: NFS1 (NFS1, Cysteine Desulfurase (NFS1))

Alternative Name: NFS1

Background: Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. Synonyms: Monoclonal NFS1 antibody, Anti-NFS1 antibody, Nitrogen fixation 1 homolog antibody, IscS antibody, NIFS antibody, HUSSY-08 antibody.

Pathways: Transition Metal Ion Homeostasis