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V5 Epitope Tag antibody

The Mouse Monoclonal anti-V5 Epitope Tag antibody has been validated for WB, ELISA, IP and DB. It is suitable to detect V5 Epitope Tag in samples from .
Catalog No. ABIN933572

Quick Overview for V5 Epitope Tag antibody (ABIN933572)

Target

V5 Epitope Tag

Reactivity

Please inquire

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This V5 Epitope Tag antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Clone

E10
  • Sequence

    GKPIPNPLLG LDST

    Purification

    Protein A affinity chromatography

    Immunogen

    V5 Tag antibody was raised in mouse using GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH as the immunogen.

    Isotype

    IgG1
  • Application Notes

    WB: 1:1000-3000
    Optimal conditions should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze/thaw cycles.
    Dilute only prior to immediate use.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
  • Target

    V5 Epitope Tag

    Alternative Name

    V5 Tag

    Target Type

    Tag

    Background

    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This tag is particularly useful for western blotting and immunoprecipitation experiments and may also find use in antibody purification.    . Synonyms: Monoclonal V5 Tag antibody, anti-V5 Tag antibody, E10 antibody, V4RR antibody, V5 antibody.
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