V5 Epitope Tag antibody
Quick Overview for V5 Epitope Tag antibody (ABIN933572)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Sequence
- GKPIPNPLLG LDST
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Purification
- Protein A affinity chromatography
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Immunogen
- V5 Tag antibody was raised in mouse using GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH as the immunogen.
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Isotype
- IgG1
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Application Notes
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WB: 1:1000-3000
Optimal conditions should be determined by the investigator. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
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- V5 Epitope Tag
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Alternative Name
- V5 Tag
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Target Type
- Tag
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Background
- The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This tag is particularly useful for western blotting and immunoprecipitation experiments and may also find use in antibody purification. . Synonyms: Monoclonal V5 Tag antibody, anti-V5 Tag antibody, E10 antibody, V4RR antibody, V5 antibody.
Target
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