Perforin 1 antibody (Biotin)

Catalog No. ABIN934012

This Mouse Monoclonal antibody specifically detects Perforin 1 in IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Perforin 1 antibody (Biotin) (ABIN934012)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: Delta G9

Product Details anti-Perforin 1 Antibody (Biotin)

Purification: Protein A affinity chromatography

Purity: > 95 % pure

Immunogen: Perforin antibody (biotin) was raised in mouse using purified granules from human YT lymphoma cell line as the immunogen.

Isotype: IgG2b

Application Details

Application Notes: FC: 2 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

This antibody recognizes the human perforin molecule of 70 kDa.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM Na0 O4, pH 7.5, with 100 M KCL, 150 M NaCl, 5 % Glycerol, 0.2 % BSA, and 0.04 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Background: Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting CD8+ cytotoxic cells upon activation. Synonyms: Monoclonal Perforin antibody, Anti-Perforin antibody.

Pathways: Apoptosis, Caspase Cascade in Apoptosis