PsbS antibody

Catalog No. ABIN93535

This Chicken Polyclonal antibody specifically detects in WB. It exhibits reactivity toward Arabidopsis thaliana, Spinach, Barley, Oat, Oryza sativa, Pinus sylvestris, Populus tremula and Rapeand has been mentioned in 1 publication.

Quick Overview for PsbS antibody (ABIN93535)

Target: PsbS

Reactivity: Arabidopsis thaliana, Spinach, Barley, Oat, Oryza sativa, Pinus sylvestris, Populus tremula, Rape

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardtii, Chlorella sp.

Predicted Reactivity: dicots and monocots, conifers

Characteristics: Expected / apparent Molecular Weight of the Antigene: 28 / 22 kDa for Arabidopsis thaliana

Purification: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from available di and monocot PsbS sequences, including Arabidopsis thaliana (At1g44575). This sequence is even conserved in conifers.

Isotype: IgY

Application Details

Application Notes: 1: 2000 - 1 : 4000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.

Storage: 4 °C

Storage Comment: store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

References

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Target Details

Target: PsbS

Background: AGI Code: At1g44575
The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence.

Molecular Weight: expected: 28 kDa, apparent: 22 kDa for Arabidopsis thaliana

UniProt: Q9XF91