RBCL antibody

Catalog No. ABIN93539

There are 9+ publications for this product available. The Rabbit Polyclonal anti-RBCL antibody is suitable to detect RBCL in samples from Arabidopsis thaliana, Soybean, Alfalfa, Pea, Synechococcus sp., Spinach, Prochlorococcus, Jatropha curcas, Porphyra, Porosira glacialis, Polytrichum formosum, Physcomytrium Patens, Phaeodactylum tricornutum, Petunia hybrida, Panicum virgatum, Nicotiana tabacum, Nicotiana benthamiana, Micromonas pusilla, Methylacidiphilum fumariolicum, Liquidambar formosana, Lathyrus sativus, Kochia prostrata, Karenia brevis, Kandelia candel, Hypnum cupressiforme, Thalassiosira pseudonana, Vitis vinifera, Verbascum lychnitis, Triticum aestivum, Tradescantia spathacea, Tomato, Tisochrysis lutea, Thermosynechococcus elongatus, Thalassiosira punctigera, Quercus ilex, Symbiodinium, Stanleya pinnata, Schima superba, Saccharum, Robinia pseudoacacia, Ricinus communis, Rhytidiadelphus squarrosus, Rape, Camellia sinensis, Agrostis stolonifera, Chlorella sorokiniana, Chlamydomonas reinhardtii, Chlamydomonas raudensis, Chickpea, Chenopodium quinoa, Chaetoceros neogracilis, Celery, Cannabis sativa L., Chlorella vulgaris, Bienertia sinuspersici, Begonia, Beet, Barley, Baculogypsina sphaerulata, Attheya longicornis, Atrichum undulatum, Artemisia annua, Apple, Fortunella margarita, Hevea, Heterosigma akashiwo, Guzmania hybrid, Gonyaulax polyedra, Gongolaria barbata, Galdieria sulphuraria, Fucus vesiculosus, Fraxinus mandshurica, Chlorococcum sp. 'dorsiventrale', Ficus, Euglena gracilis, Emiliania huxleyi, Diatom (Skeletonema), Cynara cardunculus, Cylindrospermopsis raciborskii, Cyanophora paradoxa, Coscinodiscus concinnus and Colobanthus quitensis. It has been validated for WB, EM, IL and TP.

Quick Overview for RBCL antibody (ABIN93539)

Target: RBCL

Reactivity: Arabidopsis thaliana, Soybean, Alfalfa, Pea, Synechococcus sp., Spinach, Prochlorococcus, Jatropha curcas, Porphyra, Porosira glacialis, Polytrichum formosum, Physcomytrium Patens, Phaeodactylum tricornutum, Petunia hybrida, Panicum virgatum, Nicotiana tabacum, Nicotiana benthamiana, Micromonas pusilla, Methylacidiphilum fumariolicum, Liquidambar formosana, Lathyrus sativus, Kochia prostrata, Karenia brevis, Kandelia candel, Hypnum cupressiforme, Thalassiosira pseudonana, Vitis vinifera, Verbascum lychnitis, Triticum aestivum, Tradescantia spathacea, Tomato, Tisochrysis lutea, Thermosynechococcus elongatus, Thalassiosira punctigera, Quercus ilex, Symbiodinium, Stanleya pinnata, Schima superba, Saccharum, Robinia pseudoacacia, Ricinus communis, Rhytidiadelphus squarrosus, Rape, Camellia sinensis, Agrostis stolonifera, Chlorella sorokiniana, Chlamydomonas reinhardtii, Chlamydomonas raudensis, Chickpea, Chenopodium quinoa, Chaetoceros neogracilis, Celery, Cannabis sativa L., Chlorella vulgaris, Bienertia sinuspersici, Begonia, Beet, Barley, Baculogypsina sphaerulata, Attheya longicornis, Atrichum undulatum, Artemisia annua, Apple, Fortunella margarita , Hevea, Heterosigma akashiwo, Guzmania hybrid, Gonyaulax polyedra, Gongolaria barbata, Galdieria sulphuraria, Fucus vesiculosus, Fraxinus mandshurica, Chlorococcum sp. 'dorsiventrale', Ficus, Euglena gracilis, Emiliania huxleyi, Diatom (Skeletonema), Cynara cardunculus, Cylindrospermopsis raciborskii, Cyanophora paradoxa, Coscinodiscus concinnus, Colobanthus quitensis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Electron Microscopy (EM), Immunolocalization (IL), Tissue Printing (TP)

Product Details

Purpose: Anti-RbcL | Rubisco large subunit, form I (rabbit)

Specificity: Antibody is a cellular [compartment marker] of plastid stroma or a cytopasmmarker in cyanobacteria. Antibody can detect Rubisco protein from 31.25 fmoles. Antibody will detect Rubisco Form I and Form II, therefore is suitable for workwith samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms,Raphidophytes, brown algae) as well as higher plants. This antibody can be usedto quantify Rubisco protein in plant and algal samples.

Cross-Reactivity (Details): Agostis stolonifera cv. Penncross, Arabidopsis thaliana, Apium graveolens, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata (benthic foraminifer), Beta vulgaris, Begonia sp., Bienertia sinuspersici, Brassica napus, Kandelia candel, Camellia sinensis, Cannabis sativa L., Chaetoceros furcellatus, Chlorococcum dorsiventrale, Colobanthus quitensis, Cicer arietinum, Chenopodium quinoa, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Chlorella sorokiniana, Chlorella vulgaris, Coscinodiscus concinnus, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Cynara cardunculus, Emiliana huxleyi, Euglena gracilis, Ficus carica, Fortunella margarita Swingle, Fraxinus mandshurica, Fucus vesiculosus, Gladieria sulphuraria, Glycine max, Gonyaulax polyedra, Gongolaria barbata, Guzmania hybrid, Heterosigma akashiwo, Hevea, Hordeum vulgare, Hypnum cupressiforme, Jatropha curcas, Karenia brevis (C.C.Davis) s) G.Hansen & U.Moestrup (Wilson isolate), Kochia prostrata, Lathyrus sativus, Liquidambar formosana, Malus domestica, Medicago truncatula, Micromonas pusila, Nicotiana benthamiana, Nicotiana tabacum, Panicum virgatum, Petunia hybrida cv. Mitchell, Phaeodactylum tricornutum, Physcomitrium patens, Pisum sativum, olytrichum formosum, Porosira glacialis, Porphyra sp., Ricinus communis, Robinia pseudoacacia, Rhytidiadelphus squarrosus, Saccharum sp., Schima superba, Skeletonema costatum (diatom), Skeletonema marinoi (diatom), Solanum lycopersicum, Spinacia oleracea, lichens, Stanleya pinnata, Symbiodinium sp., Synechococcus PCC 7942, Synechococcus elongatus UTEX 2973, Rhoeo discolor, Thalassiosira pseudonana, Thermosynechococcus elongatus, Triticum aestivum, Prochlorococcus sp. (surface and deep water ecotype), Triticum aestivum, dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Tisochrysis lutea, Verbascum lychnitis, Vitis vinifera, Quercus ilex
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Alpha proteobacteria,Algae (brown,red) including Galdieria sulphuraria,Arthrospira platensis,Dicots,Benincasa hispida,Kalanchoe fedtschenkoi, Beta-proteobacteria,Conifers,Cryptomonads,Cyanobacteria (prochlorophytes),Eragrostis tef,Gamma-proeobacteria,Liverworts,Manihot esculenta,Marchantia polymorpha,Miscanthus giganteus,Monocots,Mosses,Suaeda glauca,Welwitschia, Nannochloropsis sp.,Picochlorum sp.,Porphyridium purpureum,Zea mays,Zosteria marinaFor detection in Rhodospirillaceae use product AS15 2955

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide conserved across all known plant

Application Details

Application Notes: Immunofluorescence/confocal microscopy (IF), 1: 1000 (IG), 1: 250 for images see Prins et al. (2008), detailed protocol available on request, 1: 800 (TP), 1: 5000 - 10 000 and more depending upon protein load and detection method (WB)

Comment:

This antibody was used in:Immunocytochemical staining of diatoms according to Schmid (2003) J Phycol 39: 139-153 and Wordemann et al. (1986) J Cell Biol 102: 1688-1698.Immunofluorescence Dreier et al. (2012). FEMS Microbial Ecol., March 2012.Western blot and tissue printing during a student course Ma et al. (2009).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution, add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Seveso, Montano, Strona, Orlandi, Galli, Vai: "The susceptibility of corals to thermal stress by analyzing Hsp60 expression." in: Marine environmental research, Vol. 99C, pp. 69-75, (2014) (PubMed).

Scotti, Sannino, Idoine, Hamman, De Stradis, Giorio, Maréchal-Drouard, Bock, Cardi: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, (2014) (PubMed).

Kolesinski, Belusiak, Czarnocki-Cieciura, Szczepaniak: "Rubisco Accumulation Factor 1 from Thermosynechococcus elongatus participates in the final stages of ribulose-1,5-bisphosphate carboxylase/oxygenase assembly in Escherichia coli cells and in vitro." in: The FEBS journal, Vol. 281, Issue 17, pp. 3920-32, (2014) (PubMed).

Niewiadomska, Bilger, Gruca, Mulisch, Miszalski, Krupinska: "CAM-related changes in chloroplastic metabolism of Mesembryanthemum crystallinum L." in: Planta, Vol. 233, Issue 2, pp. 275-85, (2011) (PubMed).

Balsemão-Pires, Jaillais, Olson, Andrade, Umen, Chory, Sachetto-Martins: "The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism." in: BMC plant biology, Vol. 11, pp. 108, (2011) (PubMed).

Keech, Pesquet, Gutierrez, Ahad, Bellini, Smith, Gardeström: "Leaf senescence is accompanied by an early disruption of the microtubule network in Arabidopsis." in: Plant physiology, Vol. 154, Issue 4, pp. 1710-20, (2010) (PubMed).

Masuda, Mizusawa, Narisawa, Tozawa, Ohta, Takamiya: "The bacterial stringent response, conserved in chloroplasts, controls plant fertilization." in: Plant & cell physiology, Vol. 49, Issue 2, pp. 135-41, (2008) (PubMed).

Prins, van Heerden, Olmos, Kunert, Foyer: "Cysteine proteinases regulate chloroplast protein content and composition in tobacco leaves: a model for dynamic interactions with ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) vesicular bodies." in: Journal of experimental botany, Vol. 59, Issue 7, pp. 1935-50, (2008) (PubMed).

Wordeman, McDonald, Cande: "The distribution of cytoplasmic microtubules throughout the cell cycle of the centric diatom Stephanopyxis turris: their role in nuclear migration and positioning the mitotic spindle during cytokinesis." in: The Journal of cell biology, Vol. 102, Issue 5, pp. 1688-98, (1986) (PubMed).

Target Details

Target: RBCL

Background:

Background: This antibody is especially suitable for quantifying of Rubisco in plant and algal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Additional information: Anti-RbcL can be used as a cellular [compartment marker] of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies. This antibody is not suitable for use in immunoprecipiation. This product can be sold containing ProClin if requested.

Molecular Weight: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)

Gene ID: 844754

UniProt: O03042, P05698, P0C510, P00877