ExoS Gene Product (EXOS) (cleaved) antibody

Details for Product No. ABIN93557
Antigen
Epitope
cleaved
Reactivity
Pseudomonas aeruginosa (P. aeruginosa)
Host
Chicken
Clonality
Polyclonal
Application
Western Blotting (WB)
Options
Immunogen amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
Isotype IgY
Cross-Reactivity (Details) Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Predicted Reactivity Pseudomonas aeruginosa only
Characteristics Expected / apparent Molecular Weight of the Antigene: 48 kDa
Purification affinity purified
Antigen
Alternative Name Exoenzyme S (ExoS)
Background Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
Molecular Weight 48 kDa
UniProt Q51449
Application Notes 1: 5000 (WB)
Restrictions For Research Use only
Format Liquid
Buffer PBS pH 8.0+ 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
Storage 4 °C
Storage Comment store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Product cited in: Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, 2012 (PubMed). (1:5000).