H Protein of Glycine Decarboxylase Complex (GDC) antibody

Details for Product No. ABIN93559
Antigen
Reactivity
Arabidopsis thaliana, Broad Bean (Vicia faba), Spinach (Spinacia oleracea), Wheat (Triticum)
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Options
Immunogen purified GDC-H protein from Spinacia oleracea
Isotype IgG
Specificity The antibody is very specific for salmonid inducible form - hsp70.
Cross-Reactivity (Details) Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Characteristics Expected / apparent Molecular Weight of the Antigene: 16 kDa
Purification affinity purified
Antigen
Alternative Name H protein of glycine decarboxylase complex (GDC) (GDC-H)
Background The Glycine decarboxylase complex (GDC) is abundant in mitochondria of C3 leaves and functions in photorespiratory carbon recovery. GDC enzyme can account for up to 50 % of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid \\\\
Molecular Weight 16 kDa
Application Notes 1:5 000 with standard ECL (WB)
Comment

this antibody can be used on total cell extract of Arabidopsis thaliana

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL of sterile water.
Buffer PBS pH 7.4
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Storage Comment store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Supplier Images
Image no. 1 for anti-H Protein of Glycine Decarboxylase Complex (GDC) antibody (ABIN93559) Detection of GDC-H protein in Arabidopsis thaliana total leaf extract. 15 ug of a tot...