PsbO antibody

Catalog No. ABIN93573

This anti- antibody is a Rabbit Polyclonal antibody detecting in IP, IHC and WB. Suitable for Arabidopsis thaliana, Barley and Nicotiana tabacum. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PsbO antibody (ABIN93573)

Target: PsbO

Reactivity: Arabidopsis thaliana, Barley, Nicotiana tabacum

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Cross-Reactivity (Details): No cross-reactivity with: Chlamydomonas reinhardtii, Synechococcus

Characteristics: Expected / apparent Molecular Weight of the Antigene: 33 kDa

Purification: serum

Immunogen: N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 At5g66570 and 2 proteins At5g50820

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB).
Loading based on 50 - 100 ng of chlorophyll is enough to obtain good signal withthis antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL, of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Lundin, Nurmi, Rojas-Stuetz, Aro, Adamska, Spetea: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." in: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, (2008) (PubMed).

Target Details

Target: PsbO

Background: AGI Code: At5g66570
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Molecular Weight: 33 kDa

UniProt: Q9S841, P23321