Benzo[a]pyrene antibody

Catalog No. ABIN93898

This Mouse Monoclonal antibody specifically detects Benzo[a]pyrene in ELISA and ICC. It exhibits reactivity toward and has been mentioned in 2+ publications.

Quick Overview for Benzo[a]pyrene antibody (ABIN93898)

Target: Benzo[a]pyrene (BAP)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Benzo[a]pyrene antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC)

Clone: BAP-13

Product Details anti-Benzo[a]pyrene Antibody

Purpose: Anti-Benzo[a]pyrene Purified

Specificity: The antibody BAP-13 is specific for Polynuclear Aromatic Hydrocarbon and inhibits DNA adduct formation (this ability was investigated in rat liver microsomes spiked with calf thymus DNA and 7,8-diol-B[a]P).

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Benzo[a]pyrenyl-1-butyric acid conjugated to BSA.

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry: The antibody BAP-13 is suitable for immunocytochemical analysis of DNA and protein adducts of benzo[a]pyrene in tissues of various species.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Benzo[a]pyrene antibody (ABIN93898)

Scharnweber, Fisher, Suchànek, Knopp, Niessner: "Monoclonal antibody to polycyclic aromatic hydrocarbons based on a new benzo[a]pyrene immunogen." in: Fresenius' journal of analytical chemistry, Vol. 371, Issue 5, pp. 578-85, (2001) (PubMed).

Suchánek, Scharnweber, Fisher, Knopp, Niessner: "Monoclonal antibodies specific to polynuclear aromatic hydrocarbons." in: Folia biologica, Vol. 47, Issue 3, pp. 106-7, (2001) (PubMed).

Target Details for Benzo[a]pyrene

Target: Benzo[a]pyrene (BAP)

Alternative Name: Benzo[a]pyrene

Target Type: Chemical