The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Cross-Reactivity (Details)
Human
Purification
Hybridoma culture supernatant concentrated by membrane ultrafiltration.
Immunogen
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Reactivity: Human
FACS
Host: Mouse
Monoclonal
HE-10
PE
Application Notes
Immunohistochemistry (paraffin sections): The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0. Flow cytometry: Recommended dilution: 1-3 : 100.
Restrictions
For Research Use only
Format
Liquid
Handling Advice
Do not freeze. Avoid prolonged exposure to light.
Storage
4 °C
Storage Comment
Store at 2-8°C. Do not freeze.
Vanák, Drímalová, Smyslová, N?mec, Viklický, Wisniewski: "Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities." in: Neoplasma, Vol. 36, Issue 4, pp. 479-88, (1989) (PubMed).
N?mec, Drímalová, Horejsí, Vanák, Bártek, Viklický: "Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine." in: Vox sanguinis, Vol. 52, Issue 1-2, pp. 125-8, (1987) (PubMed).