Blood Group ABH antibody

Details for Product No. ABIN93921
Antigen
Reactivity
Human
5
Host
Mouse
5
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
4
3
Options
Immunogen Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Clone HE-10
Isotype IgM
Specificity The antibody HE-10 agglutinates erythrocytes of group A but does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb.
The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Cross-Reactivity (Details) Human
Purification culture supernatant
Antigen
Application Notes Optimal working dilution should be determined by the investigator.
Comment

The hybridoma culture supernatant is 4 x concentrated by ultrafiltration using 100 kDa-cut off membrane.

Restrictions For Research Use only
Format Liquid
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not use after expiration date stamped on vial label. Do not freeze.
Product cited in: Vanák, Drímalová, Smyslová, N?mec, Viklický, Wisniewski: "Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities." in: Neoplasma, Vol. 36, Issue 4, pp. 479-88, 1989 (PubMed).

N?mec, Drímalová, Horejsí, Vanák, Bártek, Viklický: "Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine." in: Vox sanguinis, Vol. 52, Issue 1-2, pp. 125-8, 1987 (PubMed).