ITGAL antibody (Integrin alpha-L)

Details for Product anti-ITGAL Antibody No. ABIN93953
Antigen
Reactivity
Human
381
160
38
13
11
10
10
9
8
6
1
1
Host
Mouse
350
114
87
7
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGAL antibody is un-conjugated
77
60
55
19
9
8
8
6
6
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)
447
133
108
98
69
67
57
48
15
13
9
8
7
6
3
2
1
1
1
1
1
1
Options
Immunogen Human peripheral blood lymphocytes
Clone MEM-83
Isotype IgG1
Specificity The antibody MEM-83 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
HLDA IV, WS Code N 211
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD11a (ITGAL Antibody Abstract)
Background CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Pathways Activated T Cell Proliferation, Integrin Complex
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Landis, McDowall, Holness, Littler, Simmons, Hogg: "Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." in: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, 1994 (PubMed).

Landis, Bennett, Hogg: "A novel LFA-1 activation epitope maps to the I domain." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1519-27, 1993 (PubMed).