CD22 antibody

Catalog No. ABIN94034

The Mouse Monoclonal anti-CD22 antibody has been validated for FACS, WB and IP. It is suitable to detect CD22 in samples from Human and Non-Human Primate.

Quick Overview for CD22 antibody (ABIN94034)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: MEM-01

Product Details anti-CD22 Antibody

Purpose: Anti-Hu CD22 Purified

Specificity: The antibody MEM-01 reacts with an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes, it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E", it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Raji Burkitt's lymphoma cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-4 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions. The antibody MEM-01 stains only the higher Mw isoform (140 kDa, migrating sometimes as a 125 kDa band) and not the lower Mw isoform (130 kDa, migrating sometimes as a 115 kDa band).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: CD22 Molecule,CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.,SIGLEC2, SIGLEC-2

Gene ID: 933

UniProt: P20273