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MYLK antibody (Internal Region)

This anti-MYLK antibody is a Goat Polyclonal antibody detecting MYLK in WB and ELISA. Suitable for Human.
Catalog No. ABIN940560

Quick Overview for MYLK antibody (Internal Region) (ABIN940560)

Target

See all MYLK Antibodies
MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity

  • 68
  • 42
  • 37
  • 2
  • 2
  • 1
  • 1
Human

Host

  • 78
  • 6
  • 3
Goat

Clonality

  • 70
  • 17
Polyclonal

Conjugate

  • 48
  • 6
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MYLK antibody is un-conjugated

Application

  • 47
  • 37
  • 30
  • 13
  • 13
  • 10
  • 10
  • 8
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA

Grade

Verified
  • Binding Specificity

    • 15
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Purpose

    MYLK

    Sequence

    KDTKNMEAKK LSKD

    Specificity

    This antibody is expected to recognise all reported isoforms (NP_444253.3, NP_444254.3 , NP_444255.3 and NP_444256.3).

    Cross-Reactivity

    Human, Mouse

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-KDTKNMEAKKLSKD, from the internal region of the protein sequence according to NP_444253.3, NP_444254.3 , NP_444255.3, NP_444256.3.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx 150 kDa band observed in Human Colon and Lung lysates (calculated MW of 197 kDa according to NP_444256.3). Recommended concentration: 0.01-0.03 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    MYLK (Myosin Light Chain Kinase (MYLK))

    Alternative Name

    MYLK

    Background

    MYLK, myosin, light chain kinase, DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK108, MLCK210, MSTP083, myosin light chain kinase, myosin, light polypeptide kinase

    Gene ID

    4638

    NCBI Accession

    NP_444253, NP_444254, NP_444255, NP_444256
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