MYLK antibody (Internal Region)
Quick Overview for MYLK antibody (Internal Region) (ABIN940560)
Target
See all MYLK AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- Internal Region
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Purpose
- MYLK
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Sequence
- KDTKNMEAKK LSKD
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Specificity
- This antibody is expected to recognise all reported isoforms (NP_444253.3, NP_444254.3 , NP_444255.3 and NP_444256.3).
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Cross-Reactivity
- Human, Mouse
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence C-KDTKNMEAKKLSKD, from the internal region of the protein sequence according to NP_444253.3, NP_444254.3 , NP_444255.3, NP_444256.3.
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Isotype
- IgG
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Application Notes
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Western Blot: Approx 150 kDa band observed in Human Colon and Lung lysates (calculated MW of 197 kDa according to NP_444256.3). Recommended concentration: 0.01-0.03 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:8000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MYLK (Myosin Light Chain Kinase (MYLK))
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Alternative Name
- MYLK
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Background
- MYLK, myosin, light chain kinase, DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK108, MLCK210, MSTP083, myosin light chain kinase, myosin, light polypeptide kinase
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Gene ID
- 4638
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NCBI Accession
- NP_444253, NP_444254, NP_444255, NP_444256
Target
-