SPON2 antibody (Internal Region)

Catalog No. ABIN940566

The Goat Polyclonal anti-SPON2 antibody (ABIN940566) specifically detects SPON2 in WB, IHC and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SPON2 antibody (Internal Region) (ABIN940566)

Target: SPON2 (Spondin 2 (SPON2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SPON2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SPON2 Antibody

Binding Specificity: Internal Region

Purpose: SPON2 (301-315)

Sequence: RTRYVRVQPA NNGSP

Specificity: Reported variants represent identical protein: NP_036577.1, NP_001121797.1, NP_001185950.1

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RTRYVRVQPANNGSP, from the internal region (near C Terminus) of the protein sequence according to NP_036577.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35-36 kDa band observed in Human, Mouse and Rat Kidney lysates, and in preliminary testing of A549 cell lysates (calculated MW of 35.8 kDa according to Human NP_036577.2,and 36.0 kDa according to Mouse NP_598664.3 and Rat NP_612542.1).

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPON2

Target: SPON2 (Spondin 2 (SPON2))

Alternative Name: SPON2

Background: SPON2, spondin 2, extracellular matrix protein, DIL-1, DIL1, DKFZp686G21139, FLJ16313, FLJ22401, FLJ34460, M-SPONDIN, MINDIN, differentially expressed in cancerous and non-cancerous lung cells 1, spondin-2

Gene ID: 10417, 100689, 171569

NCBI Accession: NP_036577