CD33 antibody

Catalog No. ABIN94072

This Mouse Monoclonal antibody specifically detects CD33 in FACS. It exhibits reactivity toward Human and Non-Human Primateand has been mentioned in 1 publication.

Quick Overview for CD33 antibody (ABIN94072)

Target: CD33

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: HIM3-4

Product Details anti-CD33 Antibody

Purpose: Anti-Hu CD33 Purified

Specificity: The antibody HIM3-4 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: NFMY-9s human cell line

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD33 antibody (ABIN94072)

McCormack, Muji?, Osdal, Bruserud, Gjertsen: "Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia." in: Blood, Vol. 121, Issue 7, pp. e34-42, (2013) (PubMed).

Target Details for CD33

Target: CD33

Alternative Name: CD33

Background: CD33 Molecule,CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.,SIGLEC3, p67

Gene ID: 945

UniProt: P20138